LIMITATION OF SEROLOGY TEST

By nfmsfaa | Updated: July 14, 2021, 7:10 a.m.

Slideshow Video

SHARE THE AWESOMENESS

Education

create your own animated videos for free

_abc cc

* Powtoon is not liable for any 3rd party content used. It is the responsibility of each user to comply with 3rd party copyright laws.

Embed code

Select a size, the embed code changes based on your selection.

_abc Powtoon Transcript

LIMITATION  OF SEROLOGICAL TEST
?
?
?
?
?
PREPARED BY
ATHIRAH NAJIHAH BINTI SULAIMAN
(2020628254)

NUR EZLYN BINTI NOZLAN
(2020879152)
NURZAIFAZATUL FARAHANUM BINTI MOHD SHUKRI
(2020449454)
lIMITATIONS IN SEROLOGICAL TESTS
 Antisera 
Availability
Red Blood Cell Source 
Neonates and immunocompromised individuals
firstly, the limitation of serological test is the availability of antisera 
01
(Moulds, 2010)
Antisera is the primary ingredient in the serological test which is used to determine which antigens are on the red cells. 
What is the function of antisera in serological tests? 
How antisera can be obtained? 
Antisera can be obtained from vertebrate animals such as rabbit, hamster, chicken, sheep, pig, goat, horse and donkey.
Antisera that are obtained from sheep, goats, pigs, donkeys and horses are expensive to maintain because they produce a large amount of antiserum. 
Delves & Roitt (1998)
While mouse produce a limited quantity of antisera. 
Because when getting an adequate pure and potent antibody from human source it should meet the criteria such as can withstand the processing technologies used in manufacturing and the stability of the shelf life  
Why is the availability of antisera limited?
(Moulds, 2010)
Second, the limitation of the serological test is red blood cell source
02
Why is red blood cell source one of the serologic test limitations? 
Research results for both the patient and the donor can be disagreeable in the red cell grouping
To determine the phenotype when recently transfused. If sensitive reading methods are used, the presence of circulating transfused cells can lead to a false-positive result
(Moulds, 2010)
How is the limitation of the approach used to determine the source of red blood cells?
A
AB
O
B
The differential centrifugation or phthalate ester results in tiny amounts of isolated red cells only. 
A method for hemolysis of red blood cells transfused with HgbA is available in HgbS (sickle cell hemoglobin) patients, but this does not provide enough red blood cells for the performance of a whole phenotypic.
(Moulds, 2010)
or heat-treated for 3 hours at 56°C.
How may false-positive reactions to human red blood cell phenotyping be avoided?
Used polyclonal reagents near their expiry date 
(Moulds, 2010)

LIMITATION OF SEROLOGY TEST

Copy this link to share with friends and colleagues:

WELCOME TO POWTOON!