DNA fingerprinting by Cassady Smith BIOCHEM2B03

By cassadyysmith3 | Updated: Nov. 15, 2022, 3:11 a.m.

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DNA Fingerprinting
By: Cassady Smith
“Hypervariable Minisatellite Regions in Human DNA.”
1984, Alec Jeffery
Discoveries 
That Aided This Development...
PCR; Kary Mullis (1983)
RFLP; Wyman and White (1980)

STR ANALYSIS
Microsatellites
STR Analysis:
Non-coding region of our Satellite DNA
2-7 base pairs in length

Number of repeats varying between individuals

Distributed abundantly throughout the genome
More reliable as only a small amount of DNA is required for this test, and the process can be done with degraded DNA
5
These are the
Steps to DNA Fingerprinting
1
Extraction
Blood
Via leukocytes or white blood cells which contain DNA
Skin cells
Skin cells such as cheek, finger, and tissue cells
Saliva
Saliva contains DNA from cells from your mouth
2
Purification/Quantification
DNA extraction methods to isolate the DNA
Examples include Chelex-100, Silica-based extraction, and the Phenol-chloroform method
Quantification, and qualification of DNA sample
Confirm it is human DNA
3
PCR Amplification
Step 2: Annealing
Step 1: Denaturing 
Step 3: Extension
Repeat steps 
4
Capillary Electrophoresis
Cathode and anode 
Capillary connecting the two
DNA travels along the capillary towards the positive anode
Shorter DNA fragments will travel faster/longer distances
Fluorescent primers excited
5
Analysis of results
Number of repeats
Number of repeats
INDIVIDUAL 1 
VERSUS
INDIVIDUAL 2
# of STRs on loci 1 from individual 1
VERSUS
# of STRs on loci 1 from indivdual 2
Future Applications
GENETIC AND INHERITED DISEASES
aided discovery in
THE END
Images and animations by PowtoonImage of Alec Jefferys from https://dnalc.cshl.edu/view/15588-Alec-Jeffreys-1989.htmlImage of inhertied diseases from https://www.statnews.com/2019/04/17/crispr-embryo-editing-ban-opposed-by-families-carrying-inherited-diseases/

DNA fingerprinting by Cassady Smith BIOCHEM2B03

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